Fiber-optic biosensor for E. coli identification

Summary form only given.The optical configuration of the experimental setup is shown. An air cooled argon ion laser was used in the experiment. The laser output power was 40 mW, and the wavelength was set at 488 nm in this arrangement. A 50 cm-long PMMA plastic fiber having a diameter of 1000 /spl mu/m was adopted as the light guide that propagated the laser beam. The fluorescence was collected by a photomultiplier (PMT) set beside the fiber. In order to separate the pumping beam (488 nm) from the fluorescence signal, a narrow band interference filter having a center frequency of 520 nm and the a bandwidth of 10 nm was fixed in front of the PMT.