High Accuracy Multi-input DNA Logic Gate Using The Spatially Localized DNA Structures

A combination of engineering and biology, using the DNA for computation, is an interesting and hopeful area. The future of this area looks very promising for diagnosis and treatment of diseases. Various DNA-based logic sensors are proposed in the past years. Recent attempts have shown that localized DNA strand displacement circuits, wherein DNA components are physically attached to a DNA surface, can improve the quality and speed of the reactions. Whereby, simultaneously run multiple instances of circuits on spatially separated locations that will be increased the speed of computation, scalability, and modularity. In this paper, we proposed a scalable method to design multiinput logic gates with spatially localized DNA hairpins that has fewer materials and speed up the desired concentration. Experimental results show that the response time and the number of required strands (fuels and anchored hairpins) are reduced more than 27% and 14%, respectively and these improvements will be more considerable in large fan-in and complex circuits.