The Method of the Formation of Cell and Tissue-engineering Structures Based on Carbon Nanotubes and Biopolymers for Tissue Restoration

In this work, a method of the formation of cell and tissue-engineering structures for regeneration of heart and blood vessel tissues was developed. Structures consisted of carbon nanotubes and biopolymers: albumin, collagen and chitosan. The device for the formation of the structures was developed based on a pulsed ytterbium laser with a wavelength of 1064 nm. The fabrication of structures was carried out by layer-by-layer laser curing of liquid dispersions of components, while the electromagnetic field of the laser led to the formation of a nanotubes scaffold in the matrix. The surface relief of each of the layers made using the developed method was investigated with an atomic force microscope. The biocompatibility of the resulting constructs was studied in vitro during cultivation of endothelial cells, fibroblasts and cardiomyocytes. The results of using the formation method are promising for obtaining structures that support tissue regeneration of cardiovascular system.