The Effect of RGD Peptide on Vascular Bone Micro-Tissue Formation

The aim of this study is to investigate the effect of RGD peptide sequence on micro-tissues formed by BMSC-HUVEC co-culture. With this aim, the RGD peptide was synthesized in the solid phase. BMSCs and HUVECs were co-cultured on agarose gel, totally 40,000 cells were used. 4 experimental groups were prepared by preparing the culture medium to 0 mg / ml, 0,5 mg / ml, 1 mg / ml and 2 mg / ml RGD. On the 1
^st
, 3
^rd
, 5
^th
and 7th days, micro-tissue images were taken under different magnification scales (4x, 10x, 20x) of a microscope and viability and size analyzes were performed. Image-J software was used for size analysis. In the results of viability and size analyzes, it was determined that the optimum RGD value which effect of the micro-tissue formed by BMSC-HUVEC co-culture was 0,5 mg / ml RGD.