The Effect of Antimicrobial Peptide Conjugated PGCL Sutures on In Vitro Wound Healing

Wound healing is a complex, vital and physiological period for supplying skin repairing after an injury or a trauma. Acceleration of the wound healing process is crucial for decreasing the morbidity and the period of the hospitalization. Sutures play major role in the closure of wounds. However, similar to other medical devices implanted in the body, sutures are foreign bodies in the wound site and serve as a nidus for bacterial attachment. Therefore, the presence of sutures significantly increases the susceptibility of a wound to infections and increases the risk of an infection in a surgical site In this case, antimicrobial peptides (AMP) which are synthesized by several organisms as first line of defense against multiple types of pathogens might be the favorable biomaterial to overcome with the surgical site infections by supporting the tissue and accelerating wound healing process. In this study, the effect of AMP conjugated poly (glycolic acid-co-caprolactone) (PGCL) surgical sutures on wound healing process was investigated by in vitro scratch assays on human keratinocyte cell lines and the antimicrobial effect was investigated on Pseudomonas Aeruginosa and MRSA.