Vascularized Spheroid Array in a Microfluidic Channel

We propose a microfluidic device which can array multiple spheroids in an extracellular matrix (ECM) gel and locate endothelial cells (ECs) on the gel with micrometer-ordered definite distance from the arrayed spheroids. The device is composed of multiple trapping sites to array spheroids in an ECM gel and a perfusable channel adjacent to the trapping sites. On the basis of the principle of flow resistance, the trapping sites can trap spheroids in every single site. Using the device, we can array multiple spheroids and ECs with the micrometer-ordered defined distances.