Point of Care Testing Chip for Multiple Virus Infection Detection Using LAMP

Here, we developed a portable microdevice for the simple and early detection of viral infections via the loop-mediated isothermal amplification (LAMP) method, as a point-of-care testing (POCT) method. This microdevice can simultaneously check 16 species of viral infection by observing fluorescence intensity, either by the naked eye or using a smartphone photo sensor. It requires only 1% of conventional sample amounts, and can run using a small battery. We also developed a simple system for simultaneously applying samples to 16 wells. Basic performance was verified using a dengue virus detection experiment, and the specific reaction was successfully confirmed following application of dengue virus RNA samples.