Deconvolution Method for Multiphoton Microscopy: An Application to Thick Ocular Tissues

Multiphoton (MP) microscopy of thick biological tissues is limited by the presence of aberrations and scattering. In addition, living samples impose an additional limit due to uncontrolled movements. To overcome these constraints, deconvolution methods are proposed as an alternative to well-established adaptive optics procedures. A marginal blind deconvolution approach for the reconstruction of MP images of both ex-vivo and living ocular tissues is shown and discussed. The quality of the images reconstructed by this method is compared to that of the original ones in terms of the achieved resolution and inherent artefacts, among other criteria.