Microchannel-Integrated Sandwich 3D Triple Electrodes for Enhanced Detection of Myoglobin as a Cardiac Biomarker

We report the development of an electrochemical redox cycling-based immunosensor using microchannel-integrated sandwich 3D triple electrode system. The triple electrodes consisting of a suspended carbon mesh and substrate-bound carbon interdigitated array (IDA) nanoelectrodes fabricated using C-MEMS. To complete an immunosensor a specific electrode was modified via the selective electrochemical reduction of aryl diazonium salts, and thus monoclonal antibody (mAb) could be immobilized on only suspended mesh electrode and the IDA electrodes were employed for the efficient redox-cycling of redox species (PAP/PQI). The microchannel integration facilitated 8-fold better low limit of detection (LOD) as compare to bulk, preventing diffusion of PAP to the bulk solution.