RainBio: Proportional Visualization of Large Sets in Biology

Set visualization is a well-known task in information visualization. In biology, it is used for comparing visually sets of genes or proteins, typically using Venn diagrams. However, limitations of the Venn diagram are well-known: they are limited to 6 sets and difficult to read above 4. Many other set visualization techniques have been proposed, but they have never been widely used in biology. In this paper, we introduce RainBio, a technique for visualizing sets in biology and aimed at providing a global overview showing the size of the main intersections, in a proportional way, and the similarities between sets. We adapt rainbow boxes, a technique for visualizing small datasets, to the visualization of larger sets, using element aggregation and intersection clustering. We present the application of RainBio to three datasets, with 5, 6 and 12 sets. We also describe a small user study comparing RainBio with Venn diagrams, involving 30 students in biology. Results showed that RainBio led to significantly fewer errors on 6-set dataset, and that the majority of students preferred RainBio. RainBio is proposed as a web-based tool for up to 15 sets.