A New Process Using Magnetic Nanoparticles to Neuronal Cells Growth Orientation

Degenerative neural diseases after accidents are important matters in neuroscience field. Physical stimulus for neuronal growth and development can be achieved with tensile force. This experimental method consists of using magnetic nanoparticles attached to neuronal cells on which static magnetic field is applied to stimulate targeted cells' growth in the field's direction. Particles under a force of magnetic field can provide physical guidance for neuronal regeneration. Particles under a force of magnetic field can provide physical guidance for neuronal regeneration. Influence of particles' concentration and intensity of the field was analyzed to determine the optimum values for higher oriented growth by processing the images data obtained using electronic microscopy. Higher directed growth of neuronal cells with MNPs was observed from qualitative and quantitative evaluation of images data, obtained in the experiments. Consistent and reliable results were achieved using data mining technique. The nanoparticles functionalization was made with less expensive biomaterial. The particles were homemade and they are biocompatible. The magnetic field was applied with magnets. This new experimental methodology is less costly than others found in the literature. The described technical characteristics combined made this work a new, simple and low cost strategy to stimulate neuronal cells oriented growth. Furthermore, this method shows viability for larger researches to develop therapies for recovery of neurodegenerative diseases, avoiding amputation.