Investigation of the Electrical Stimulation Effect on Cell Growth on Bioengineering Nanocomposite Substrates

Method of electrical stimulation of cells on bioengineering nanocomposite substrates with electrical pulses with amplitude from 60 mV to 120 mV and pulse duration of 2.5 ms for 24 h is developed. Nanocomposite substrates consist of a polymer matrix based on natural components - protein albumin, aminosugar chitosan and reinforcing filler - carbon nanotubes have a three-dimensional structure with an internal electrically conductive frame created by laser formation. A developed device for electrical stimulation of cells allows the transmission of stimulating signals to cells through a conducting substrate consisted of bioengineer nanocomposite. As a result, the dependence of the proliferation factor on the electric signal amplitude was obtained and characteristics of the spreading of cells on the substrate was investigated.