Stichopus Hermanni Collagen with Local Hydroxyapatite as Bone Substitute Material Toward Osteoclast Number and Toxicity

Denture wearing is very important after tooth extraction, because it can rehabilitate-mastication, phonetics and aesthetics. After tooth-extraction, alveolar bone resorption disturbs the stability and retention of denture. Hydroxyapatite has several weaknesses and high bone density. Stichopus hermani collagen is a product of gold sea cucumber collagen extraction which contains 80% collagen. The purpose was to observe the effect of Stichopus hermanni collagen with local hydroxyapatite as bone substitute material toward osteoclast number and toxicity.The subjects were 75 male of Rattus Sprague Dawley, each was 3 months old. The subjects were divided into 3 groups. Group I was Stichopus hermanni collagen and local hydroxyapatite, group II was Stichopus hermanni collagen, and group III was collagen. Each of the subject was decapitated after 3, 7, 14, 28, 56 days after treatment. The defect was made on the femur condyle of Rattus Sprague Dawley. The histology slides were made from defect area. The trinocular microscope was used to measure osteoclast. The data were analyzed using the two-ways ANOVA test. The tests of hepatic and renal toxicity were done by making histology slides measured with trinocular microscope. The data were analyzed using Kruskal Wallis test. Result: The two-ways ANOVA test showed there was significant difference among group of Stichopus hermanni collagen and local hydroxyapatite, group of Stichopus hermanni collagen, and group of collagen after 3, 7, 14, 28, 56 days of treatment on the number of osteoclast (p<;0.05). The Kruskal Wallis test showed there was no significant difference between group of Stichopus hermanni collagen and local hydroxyapatite, and collagen after 3, 7, 14, 28, 56 days of treatment on the hepatic and renal toxicity (p>0.05). Conclusion: Stichopus hermanni collagen with local hydroxyapatite as bone substitute material increases osteoclast compared with Stichopus hermanni collagen, and collagen. Stichopus hermanni collagen with local hydroxyapatite does not cause systemic toxicity.