Detection of Multiple Embryo Growth Factors by Bead-Based Digital Microfluidic Chip in Embryo Culture Medium

In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL-1β and TNF-α in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead-based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-α calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL-1β calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.