Acquisition of 3D Natural Scaffold by Decellularization

Regenerative Medicine and Tissue Engineering has been gaining critical importance in the medical sector, specifically in cancer and organ-tissue losses. Laboratory studies have been conducted on tissue and organ generation using whole organ decellularization and recellularization techniques. In this study, whole rat pancreas has been decellularized by perfusion and imaged using confocal microscopy. Whole rat pancreas tissue has been divided into control and exerimental groups and decellularized using hypotonic, hypertonic and detergent solutions. The tissues, imbedded in Tissue Tech and frozen at -20 , have been prepared as 30 micrometer sections, dyed using DAPI for confocal microscopic imaging of the cell nuclei. It has been demonstrated that the cell nuclei imaged in the control group have been removed as a result of the decellularization process. These findings indicate that the collagenous tissue has been preserved to a large extent. With the imaging of the serial sections, 3D reconstruction of decellularized pancreas can be obtained.