OCT-based label-free 3D mapping of lymphatic vessels and transparent interstitial-fluid-filled dislocations

Approach to OCT-lymphangiography (OCT-LA) and interstitial-fluid-filled dislocations mapping that complement speckle variance (SV) OCT angiography is presented. OCT-LA can be extracted from the same OCT raw datasets that were acquired for SV OCT angiography. Lymphatic vessels and interstitial dislocations are filled with transparent fluids (e.g. lymph) and can be separated from the blood vessels and tissue. In vivo application of the OCT-LA approach is demonstrated on NRG mouse with grown BX-PC3 tumor (human pancreatic cancer cells).