A Centrifugal Microfluidic Platform to Measure Hemoglobin of Whole Blood

Microfluidics has been becoming more and more popular over last two decades. The reason for this, is inherent features of microfluidics including low consumption of samples and reagents, high sensitivity, short analysis time, and low cost. As well as, centrifugal microfluidics as a subset of microfluidics has been able to prove itself as a helpful tool in analytic assays. The main application of centrifugal microfluidic devices is being used in point-of-care testing systems. Herein, we presented a microfluidic disc for measuring hemoglobin(Hb) concentration in the EDTA-anticoagulated venous blood using cyanmethemoglobin method. In this experiment, at first a hand-made standard solution was made and using it, a linear standard curve was plotted. In order to detect of Hb concentration, we designed two inlets, one mixing chamber, and a detection chamber. In our design, the blood and Drabkin's solution due to the displacement between primary and secondary mixing chambers were mixed well and all erythrocytes were lysed completely. As well as, we designed the detection chamber in a way that, it is filled with fluid using inertial force without any bubble trapping. Then, the absorbance of samples was read out with a high-resolution spectrometer. Finally, in order to prove the assay procedure, the hemoglobin concentration of 11 blood samples was quantified. The results were compared with hematology analyzer data. The quantified values had good linearity and also good conformity with hematology analyzer data. In this experiment, the maximum standard deviation and the required time were ± 0.51 and 68 seconds, respectively.