Simulation of Electroporation in Cell Using Bipolar AC Pulse

Electroporation in a single cell was numerically simulated using bipolar AC electric pulses. A cell of 20 μm in diameter was placed in the center of a microchannel with 100 μm width and height and AC sine pulse with 2kV/cm electric field and 100 kHz in frequency were applied on the electrodes at the top and bottom of the microchannel. Our obtained simulation results show that most nanopores are created around the poles of cell. The transmembrane potential would be influenced by the shape of the external applied voltage. Additionally, the numerical simulation also demonstrate the conductivity of porated area increase tremendously compared to that of the non-porated area.