Transendothelial electrical resistance (TEER) measurement system of 3D tubular vascular channel

This conference proceeding reports a system for measuring transendothelial electrical resistance (TEER) of 3D tubular vascular channel as an index of endothelial barrier integrity. We introduced an electrodes-pair into the channel inside a collagen sgel in a culture device, and set a counter electrodes-pair parallel to the channel (Fig. 1). By connecting the electrodes with a volt-ohm meter, TEER measurement of the vascular channel coated with endothelial cells (HUVECs) was achieved. Moreover, by perfusing the channel with culture medium via the connection of the device, the influence of the perfusion on the TEER was measured during cultivation. According to these results, we believe that our system will be a useful platform for drug permeability assay utilizing the 3D channel.