A Simulation Study on Light Scattering Effect on Water-borne Bacteriophage Virus Using Mie Analysis

Diagnosis and characterization of Viruses, one of the most critical particle type among the water-borne organisms, have been going through continuous process of improvements and analyses. In this paper, a Mie scattering analysis has been presented for optical sorting of nanoscale sphere shaped Y1, T4 and C2 bacteriophage viruses suspended in Potassium Bi-Phosphate (PBS) solution. In this simulation work, Bohren and Huffman (BHMIE) model has been used and the incident light of 514 nm wavelength (resembling Argon ion laser) has been considered. Intensity components for different angles have been studied and it has been found that from 30º angle the components for the three viruses shift their comparative positions and give different characteristics. The extinction and scattering efficiencies remain same and around zero from size factor 0 to 0.6. But after size factor 0.6, the organism with smallest diameter shows the highest efficiency, whereas the other two viruses show identical efficiency result.