In vitro cell culture study of the conditions of inhibition effect of tumors under alternating electric field

In vitro cell culture device was developed for the investigation of inhibition effect of cell colonies using alternating electric field. Cancer cells were cultured in 3D hydrogel and cell colonies, or called tumor spheroids, were gradually formed in the culture device. During the culture course, alternating electric field was applied and the conditions were investigated to optimize the inhibition effect of cell colonies. Results showed that electric field at frequency of 130 kHz and 0.5 Vpp was the minimal effective conditions of the inhibition of cell colonies. The device was demonstrated to provide a practical tool for the investigation of the efficacy and the therapeutic conditions of inhibiting tumors.