Lab-on-chip stretchable impedance spectroscopy device for mammalian cells studies

This paper presents the fabrication and testing of a novel Electric Cell-substrate Impedance Spectroscopy (ECIS) biosensor. This is the first time when ECIS electrodes were fabricated on a stretchable polydimethylsiloxane (PDMS) substrate and ECIS measurements on mammalian cells exposed to cyclic strain of 4% and 8% were performed. The stretchable ECIS biosensors simulate in vitro the dynamic environment of organism, such as pulsation, bending and stretching, which enable the investigation on cell behavior that undergoes mechanical stimuli in biological tissue. This novel stretchable ECIS sensor was demonstrated to measure the cell impedance in static and stretching states.