Development and testing of portable fluorescence reader (PorFloR™)

PorFloR™ is a point-of-care portable fluorescence reader. It is a small handheld device that records the intensity of fluorescence spot and change in fluorescence intensity of the spot on the solid 2D base (e.g. paper strips) material. Nowadays, numerous fluorescence based lateral flow immunoassay (LFIA) techniques are utilized for the detection of various disease biomarkers. Most of the techniques depend on visible colorimetric detection process. Results obtained from such techniques are qualitative or semi-quantitative. Thus, we have developed the portable, easy-to-use, low cost, point-of-care strip reader that can detect the appearance as well as change in fluorescence intensity on the surface with quantitative precision. The output from device can be programmed to display the presence as well as concentration of an analyte.