A bone marrow cavity segmentation method using wavelet-based texture feature

A better understanding of in vivo bio images is expected to contribute to the discovery of new drugs and mechanisms of disease. To improve the contributions of in vivo bioimaging, the extraction of a particular region is required in order to detect a particular cell's motion because manual image processing of a massive number of images is unrealistic. One of the issues for automatic image-segmentation is that conditions of image-taking are variable. Thus, some manual input and/or manual tuning of some parameters is required to adjust each image. To reduce manual operation for image processing of bone marrow cavity segmentation, we focused on the texture pattern of bone marrow cavity. In this paper, we propose a bone marrow cavity segmentation method using support vector machine and wavelet-based texture feature. The proposed method does not require manual inputs to obtain distribution of intensity before processing, because the texture patterns of bone marrow cavity regions are integrated into the system in advance. Moreover, it is applicable to a particular frame in an image sequence in which the condition of fluorescent material is variable because it does not require temporal variation or initial frame for the segmentation. In the experiment, we evaluated our method with nine types of mother wavelets and several sets of scale parameters. The bone marrow cavity segmentation, using graph-cuts with our texture pattern classification, performs well without manual inputs by a user.