Investigation of pulsed electric field on cancer cell cultured on patterned surface

Electroporation (EP) is a method of controlling cell function by using pulses of electrical fields to create a pore through a cell membrane and causes other substance around it to be absorbed into the cell. Where this method had been led to a variety of medical applications. In this study, we will use voltage of electroporation is 2000V/cm with pulse electric field of 30μs and single pulse to the HeLa Cell (Human Cervical cancer cell) that used as a primary cell in this study. Other than that, we also study the effect of protein surface with parameter of pulsed electric field mention above. Which are the comparison effect of HeLa cell with protein surface and an empty surface of substrate In certain cases, it is important for the cell to be in the specific location. Which also we studies on microcontact printing (μCP) method, it is a quite useful technique for patterning extracellular matrix as an adhesion molecule for cells that works for controlling the cell growth. By this two method which is EP and μCP method, we will combine this with the method in an experiment in order to see the effect of EP technique on cell properties cultured on micropatterned surface.