A highly sensitive Amyloid-β detection by cantilever microsensor immobilized with liposome with incorporated cholesterol and phosphatidylcholine lipid with short hydrophobic acyl chains

We improved the sensitivity of NiCr strain gauge cantilever immobilized with liposome to detect Aβ protein with low concentration. In this work, by immobilizing liposome incorporated cholesterol on cantilever, we could detect 50-nM Aβ(1-40). Also, to investigate whether the hydrophobic carbon number of acyl chains of hydrophobic group influences Aβ-membrane interaction, DMPC, DPPC, and DSPC liposomes were immobilized, respectively, on the cantilever surface. The results show that DMPC-Aβ interaction is much larger than DPPC-Aβ and DSPC-Aβ interactions. We expect that the cantilever microsensor can apply to detect the Aβ(1-40) protein in patients of Alzheimer's disease (AD) and also Mild Cognitive Impairment (MCI).