Image analysis for subcellular microfluorimetry: Examples of application in single yeast cell studies

Computer image processing algorithms of the ImageJ software (National Institutes of Health, USA; http://rsb.info.nih.gov/ij) have been tailored for subcellular microfluorimetry of the single Saccharomyces cerevisiae yeast cells. The algorithms were used for quantitative analysis of the digital images acquired by the wide-field fluorescence microscopy combined with color digital photography aiming to investigate the intracellular distribution of anthracycline anticancer drug doxorubicin and to evaluate intravacuolar viscosity.