Improved result for the refractive index of human hemoglobin solutions by Kramers-Kronig relations

Applying Kramers-Kronig relations we compute the real part of the refractive index of aqueous solutions of human hemoglobin from its absorption spectra in the range 250 nm–1100 nm. Since the solution's absorption is not limited to this spectral range, strong ultraviolet (UV) and infrared absorbance of the water have been considered in previous investigations. We improve these results regarding the concentration-dependent absorption of water, the UV absorbance of hemoglobin's peptide-backbone, and by fixing the remaining unknown parameters via a global fit.