Insilico screening of Prevotella Intermedia 17 identifies Lipopolysaccharide Biosynthesis Pathway genes as potential drug targets

Periodontal disease is an infectious disease caused by gram negative bacteria. The aim is to identify the potential drug target of Prevotella Intermedia strain 17. In silico analysis was performed to find the essential genes in Prevotella Intermedia strain 17. 470 protein sequences was retrieved from NCBI, 439 sequences passed the 90% threstold in CD-HIT, BLAST filtered 24 sequences as homolog to human and retained remaining 415 sequences. DEG identified 144 proteins as essential genes. Ezypred classified 57 essential genes as enzymes and 87 as non enzymes. Psortb identified the cellular component of the essential genes where most of the genes was found to be cytoplamic. KEGG identified the genes involved in the unique pathways of Prevotella Intermedia strain 17. Two enzymes 3-deoxy-D-manno-octulosonate cytidylyltransferase and 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase were identified to be involved in Lipopolysaccharide Biosynthesis Pathway which is a unique pathway for gram negative bacteria.