Portable Electrowetting Digital Microfluidics Analysis Platform for Chemiluminescence Sensing

A prototype of low-cost compact digital microfluidics analysis platform controlled by a smartphone was built. Controlled by a DIY-built application (App) with self-calibration, the smartphone was applied not only as the controller that manipulated droplets on an electro-wetting-on-dielectric microfluidic device through our home-made circuits, but also as the reader that quantified chemiluminescence signals through its camera. In order to deal with the non-uniformity of the chemiluminescence images acquired by the smartphone, we proposed a customize image process scheme. The results showed linear responses to H2O2 from 1 to 20 mM in the luminol-HRP-H2O2 chemiluminescence system.