The effect of voltage sensitive dye di-4-ANEPPS on the RT/RR coupling in rabbit isolated heart

Voltage sensitive dye (VSD) di-4-ANEPPS is ordinary used for optical measurement of action potential in experimental cardiology. In this work, the effect of the dye on the RT/RR coupling measured in electrograms (EG) recorded on rabbit isolated hearts was studied. The results were compared with the control experiments without VSD administration. The comparison was performed in preparation stage of the experiment that consisted of three phases: stabilization (S), VSD loading (L), and washout (W).