Kinetic model reconstruction in silico of NSD metabolism network

Basing on the kinetic model analysis of cell metabolism network can visualize the movement of intracellular metabolism, and provide the link between cell culture and manufacture of biological products. The kinetic model reconstruction in silico of nucleotide sugar donor (NSD) metabolic network has been put forward by Philip et al., which involved with 34 metabolites mass balances and 60 metabolic reaction rates. In this paper, the NSD metabolic network kinetic model is partly modified basing on the Philip's model. 26 significant kinetic parameters are estimated again by the genetic algorithm (GA) in this model. Compared to the traditional methods, the better simulation results are obtained in new kinetic parameters for metabolite concentrations. It offers an optimizable platform for the quality control of biological products.