Investigation a critical selection of pulse duration effect on growth rate of HeLa cells

Electroporation is a process of the biophysical effect on cells exposed to an external electrical field is gaining applications in medical treatments, especially to create pores through a cell membrane and allow uptake of DNA into a cell. Therefore, in the theoretical evaluation of electroporation, transmembrane potential and characteristics of the cells growth is the target of the analysis. In this study, we measured the relation between amplitude and duration, used cervical cancer cells (HeLa cells) to be sample for electroporation, because HeLa cells is one of the most well-known cell lines, and easy to researched by continuous harvesting of large numbers of HeLa cells for in-vitro experimental tests. Cells were submitted to single pulses, at constant field strength of 2kV/cm, at various pulse durations from 30µs to 600µs. As a summary, it was found pulse duration equal to100µs is associated the HeLa cell growth rate faster confluence in comparison to the cell with 30µs, 300µs and 600µs pulse duration.