Using magnetic marked PEGDA-based cell sheets for three dimensional lobule-mimicking chip

The formation and regeneration of tissue are the result of intricate temporal and spatial coordination of numerous individual cell fate processes. It is regulated not only by cell autonomous processes but also by extracellular microenvironmental stimuli. In this study, we propose lobule-mimicking culture chips by using magnetic-mark poly (ethylene glycol) diacrylate (PEGDA)-based cell sheets (MP cell sheets). It consists of four cell sheet chambers and one stacking chamber. MP cell sheets were formed by UV light two times. Using magnetic field to manipulate MP cell sheets moving and rotating. Finally, we had stacked MP cell sheets to achieve lobule-mimetic regeneration.