On Line Monitoring Of Glucose, Lactate, Glutamine, And Glutamate During Mammalian Cell Cultivations With Integrated Micro Biosensor array

A sensorchip with an integrated flow cell with a volumina of 1 /spl mu/l provided the possibility of simultaneous measurement of four different parameters. Integrated thin film biosensors for simultaneous measurement of glucose, lactate, glutamine, and glutamate were combined to a biosensor array. Due to a novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed during mammalian cell culture.