Aptamer immobilization on MWCNT-modified SGFET for detection of HIV-1 Tat protein

A solution gate field effect transistor (SGFET) has been fabricated for the detection of HIV-1 Tat protein. Functionalized carboxylated multiwalled carbon nanotube (COOH-MWCNT) has been deposited on the FET to act as amplification layer and immobilization site for aptamer. Aptamer has been a very reliable probe for detection of protein with high specificity. In this paper, we demonstrated the immobilized aptamer on MWCNT-modified SGFET. The bond formation and surface morphology of MWCNT were examined using FTIR and FESEM, respectively. The fabricated device shows the current decreases due to resistance increased after every immobilization step. The implementation of aptamer as a recognition element is promising for HIV-1 Tat protein detection.