Circulatory culture system for multilayer-structured tubular tissues

We proposed a 3D assembly technique and a circulatory culture system. The technique gives multilayer-structured tubular tissues for blood vessel models. By the circulatory culture system, this study was aimed to determine whether the tissue-engineered tubular models maintain the normally mechanical property as the development and function. In this work, we were able to fabricate tubular tissues from multilayered tissues rapidly in the suitable conditions and circularly culture the fabricated multilayer-structured tubular tissues.