Nanoplasmonic biosensing platform for multiple pathogen detection

In this study, we present for the first time a reliable, fast, accurate and sensitive multiple pathogen detection platforms that can be adapted to the point-of-care (POC) in resource-constrained settings. Here, nanoplasmonic properties of metal nanoparticles are utilized to detect the captured viruses on the biosensing surface, and the platform also allows to report a quantitative viral load for HIV-infected anonymous discarded patient whole blood samples. The limit of detection covers the definition of treatment failure indicated by World Health Organization (WHO) (viral load (VL)>5,000 copies/mL) and the Department of Health and Human Services (DHHS) and AIDS Clinical Trials Group (ACTG) (VL>200 copies/mL).