Immobilization of BSA on the polymethacrylic acid

N, N'-Methylenebisacrylamide was used as the crosslinking agent to prepare the polymethacrylic acid and its carboxyl groups were modified by N-(3-Dimethy-laminopropyl)-N'-ethyl carbodiimide hydrochloride and N-Hydroxy succinimide. The ability of immobilizing BSA and the influence of reacting time of carrier were studied. The immobilization amount and the optimal reacting time were 2100mg/g and 90 minutes respectively. The carrier was characterized by infrared detection. The results indicated that the polymethacrylicacid possessed good performance and the ability to immobilized BSA was enhanced after the modification of EDC-NHS.