Microelectrode arrays for electrophysiological monitoring of hippocampal organotypic slice cultures

A three-dimensional platinum (Pt) microelectrode array embedded on a micromachined silicon (Si) substrate (porosity of 13%, via hole diameter of 40 /spl mu/m) has been developed. Electrodes are 35-/spl mu/m wide and 20-/spl mu/m high, spaced 200 /spl mu/m apart and arranged in an elliptic geometry. Integrated within a microperfusion chamber, the devices were used for stimulation and recording experiments of hippocampal slice cultures over a period of several days.