Extraction and Preliminary Purification of Cellulase from Cipangopaludina Cahayensis

Cellulose is the largest renewable resource in the world, and it has the highest utilization value in biomass energy. But the biggest problem in usage of cellulose is degradation, the most effective solution is to make use of cellulase to degradated it, which is thorough and free from contamination. With gradually increased understanding of cellulase, more and more people start to study the cellulase from animals. In this paper, methods of cellulase extraction from Cipangopaludina Cahayensis were studied. Effects from extraction temperature, time, pH and extraction buffer on extraction result were studied and optimal conditions were determined. Moreover cellulase activity was measured and cellulase were purified preliminary by sulphat precipitation and dialysis. All these will be a new way on cellulose energy-oriented use.