Influence of 60 ns pulsed electric fields on embryonic stem cells

Influence of pulsed electric fields on mice embryonic stem cells was investigated. The pulsed power system used in this experiment had a pulse width of 60 ns, with an electric field varying from 10 to 30 kV/cm, a current varying from 200 to 600 amperes and a repetition rate of 1 pulse per second. Results show that the pulsed electric field suppresses ES cell proliferation, while the undifferentiated marker expression is retained during proliferation.