2-dimensional separation of biomimetic particles by stopped-flow centrifugo-magnetophoresis

This paper presents a centrifugo-magnetophoretic principle for the 2-dimensional separation of particles towards cell separation applications. Multi-force particle separation is achieved through the favorable combination of centrifugal sedimentation and magnetic deflection, while a stopped-flow mode (i.e., no fluid flow, mere sedimentation) is implemented to minimize divergent flow-lines and hydrodynamic instabilities typical in pressure-driven microfluidic platforms. The results demonstrate immuno-capture of biomimetic elements by magnetic particles and subsequent routing of these particle tandems to a designated outlet. During separation, shear stresses and forces that can unfavorably alter biological cells are reduced as compared to more common pressure-driven systems. Hence, the novel, stopped-flow, centrifugo-magnetophoretic separation system enables a possible post-separation analysis of mechanically undisturbed cells.