Cloning of DREB gene from Lilyturf (Ophiopogon japonicus) and construction of a plant expression vector

The cDNA of DREB transcription factor named OjDREBl was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREBl is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure of OjDREBl contains a single AP2 domain and shows the typical characteristics of DREB gene family. Homological analysis showed that 73% amino acids in the sequences is similar to Asparagus officinalis DREB3. The OjDREBl gene was cloned into the PBI121 vector to obtain plant expression vector .