Reprogramming of cells using modified mRNA

Reprogramming of cells for delivery of proteins at specific sites holds great promise for future clinical applications. Chitosan delivery agents in form of nano-spheres presents a readily available biodegradable option for controlled release of mRNA in the cytosol of the cells. In this work we are using Enhanced Green Fluorescent protein as a proof of concept to show for transfection of osteoblast cells with modified mRNA. Transfection was carried out using lipofectaime RNAiMAX.