Active virus filter for enrichment and manipulation of virus

We developed an active virus filter (AVF) that was able to virus enrichment and distribution for single virus manipulation by using the insulator based dielectrophoresis (iDEP). The design of constricted flow channel enabled the microfluidic chip to produce iDEP force. We utilized maskless photolithography to achieve the precise 3D gray-scale exposure for constricted flow channel. When we applied sinusoidal wave to the electrodes within the range of an amplitude of 20 Vp-p and frequency of 10 MHz. AVF achieved enrichment of influenza virus (A PR/8) by the positive dielectrophoretic force.