Synthesis and Identification of Artificial Antigen for Bensulfuron-Methyl

In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with BSM-OVA to BALB/c mice, high titer and sensitive polyclonal antibodies against bensulfuron-methyl herbicide were obtained. The titer was 1:1.28 × 10
⁵
. The IC
₅₀
was 0.032 μg/mL. The results indicated that the artificial antigen for BSM synthesized successfully, and it could be used to produce monoclonal antibodies for BSM.