Effects of Water-Borne Copper on Isozymes of Gill in Eriocheir sinensis

By using the PAGE (Poly Acrylamide Gel Electrophoresis), the effect of water-borne Cu
²⁺
on the isozymes in gills of E. sinensis was studied. The experimental groups were treated with different concentration of Cu
²⁺
as 0.01, 0.10, 1.00, and 5.00 mg/l, respectively. The results showed that different isozymes had specific responses. The activity of LDH and MDH decreased gradually along with the increasing of Cu
²⁺
level. Increasing of Cu
²⁺
level followed by decreasing of ALP was observed, and the variation of EST wasn't significant. These isozymes variations reflected the toxic effects of Cu
²⁺
sensitively.