Development of an Acetic Acid Microbial Biosensor for Environmental Monitoring

An acetic acid microbial biosensor was developed for environmental monitoring. The microbial biosensor consisted of an immobilized cell membrane and an oxygen electrode. The results showed that the optimum pH of buffer solution was 6.48, the optimum temperature of buffer solution was 33°C and the optimum concentration of buffer solution was 0.01mol/L for acetic acid's determining. The linear range was found to be from 5.0 to 30 mg/L of acetic acid and the regression equation was y=0.51x+1.66 (correlation coefficient 0.9998). 23 kind of interferential substances, which have the similar chemical characters and molecular weights as acetic acid, were determined in the experiment. The biosensor was found to have an extend activity of 90 days with more than 600 determinations.