Ultrasound mediated gene silencing with short-hairpin RNA

Ultrasound-mediated delivery (USMD) of drugs and genes, especially with the enhancement of microbubble or nanoparticle agents, has been demonstrated in various settings for some years. Translation to pre-clinical disease models faces many challenges. This paper reports on the development and characteristics of a device for preclinical translational research in USMD. Feasibility of short-haripin RNA (shRNA) delivery in vivo using this device was demonstrated.